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Screening Multiple Soybean Cultivars (MG 00 to MG VIII) for Somatic Embryogenesis Following Agrobacterium ‐Mediated Transformation of Immature Cotyledons
Author(s) -
Ko TaeSeok,
Nelson Randall L.,
Korban Schuyler S.
Publication year - 2004
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2004.1825
Subject(s) - biology , somatic embryogenesis , cultivar , transformation (genetics) , agrobacterium , hygromycin b , explant culture , cotyledon , botany , horticulture , gene , genetics , in vitro
A total of 15 soybean [ Glycine max (L.) Merr.] cultivars representing maturity groups 00 to VIII were assessed for their embryogenic and transformation potentials. All cultivars were identified as embryogenic under hygromycin selection following Agrobacterium ‐mediated transformation of immature cotyledons. Histochemical β‐glucuronidase (GUS) assays of induced hygromycin‐resistant somatic embryos (SEs) showed that 13 out of 15 cultivars were amenable for transformation. Wide variations among different genotypes were observed for their embryogenic capacity under hygromycin selection. Three cultivars, Cisne, Council, and Kunitz, were highly embryogenic yielding more than 50% responding explants and 1.5 to 2.4 hygromycin‐resistant SEs per responding explant. The transformation potential of multiple soybean cultivars was highly correlated with the embryogenic potential of immature cotyledons under hygromycin selection. It was possible to distinguish between highly and poorly embryogenic genotypes by visually observing the phenotype of cultured immature cotyledons. For highly embryogenic cultivars, the induction of somatic embryos mainly originated from actively dedifferentiating and browning/necrotic tissues along the margins of the abaxial side of cultured cotyledons.