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Identification of Molecular Markers Linked to the Fertility Restorer Genes for CMS‐D8 in Cotton
Author(s) -
Zhang Jinfa,
Stewart James McD.
Publication year - 2004
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2004.1209
Subject(s) - rapd , bulked segregant analysis , biology , genetics , molecular marker , genetic marker , gossypium , genome , gene , gene mapping , population , chromosome , genetic diversity , sociology , demography
The identification of molecular markers closely linked to restorer genes of the cytoplasmic male sterile (CMS) D8 system could facilitate the development of parental lines for hybrid cotton ( Gossypium hirsutum L.). Our objective was to develop molecular markers closely linked to the two independent dominant restorer genes, Rf 1 from the D2 restorer line transferred from G. harknessii Brandegee (D 2 genome) and Rf 2 from the D8 restorer line. Bulked segregant analysis was used to identify random amplified polymorphic DNA (RAPD) markers that were linked to the restorer genes. Three testcrosses were used for mapping Rf 2 and two for Rf 1 relative to the markers. Two RAPD markers, UBC111 3000 and UBC188 500 , were associated with Rf 2 in coupling phase. UBC188 500 was closely linked to Rf 2 with an average genetic distance of 2.9 cM. A survey of cotton species and cultivars revealed that UBC188 500 was absent in normal cotton cultivars and most Gossypium spp. However, it was present in the D8 restorer line, G. raimondii Ulbr. (D 5 ), G. trilobum (DC.) Skovst. (D 8 ), and selected wild species from Australia, indicating that Rf 2 and the DNA fragment yielding the RAPD marker were both introgressed into the tetraploid cottons from the D 8 genome. A new RAPD marker, UBC169 700 , and a previously identified RAPD marker, UBC659 1500 , cosegregated with Rf 1 in the two populations examined. The three RAPD markers, UBC188 500 , UC169 700 , and UBC659 1500 were converted into reliable and genome‐specific sequence tagged site (STS) markers on the basis of their sequence information. These markers are restorer‐specific and should be useful in marker‐based selection for developing restorer parental lines and constructing a high‐resolution linkage map containing Rf 1 and Rf 2