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Rps 8 Maps to a Resistance Gene Rich Region on Soybean Molecular Linkage Group F
Author(s) -
Gordon Stuart G.,
St. Martin Steven K.,
Dorrance Anne E.
Publication year - 2006
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2004.04-0024
Subject(s) - phytophthora sojae , biology , restriction fragment length polymorphism , genetics , locus (genetics) , genetic linkage , population , gene , quantitative trait locus , gene mapping , plant disease resistance , genetic marker , genotype , demography , sociology , chromosome
Phytophthora root and stem rot caused by Phytophthora sojae M.J. Kaufmann & J.W. Gerdemann is a serious disease of soybean [ Glycine max (L.) Merr.] worldwide. Recently, a new locus for resistance to P. sojae , Rps 8, was identified and mapped in two small soybean populations. The objective of this study was to verify the genomic location of Rps 8 in a larger population. One hundred thirty‐eight F 2:3 families from a cross between ‘Williams’ ( rps 8/ rps 8) × PI 399073 ( Rps 8/ Rps 8) were genotyped by simple sequence repeat (SSR) and restriction fragment length polymorphism (RFLP) markers. From this set of families, 138 and 69 were phenotyped with P. sojae races 1 and 25, respectively. The segregation ratio for each race fit a 3:1 resistant:susceptible, confirming that Rps 8 segregated as a single dominant gene. On the basis of linkage analysis with SSR and RFLP markers, Rps 8 was located on molecular linkage group F in this population. This region of the soybean genome contains numerous other resistance gene loci as well as pathogen and pest resistance QTL.