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Microsatellite Markers Flanking the tms2 Gene Facilitated Tropical TGMS Rice Line Development
Author(s) -
Lopez M. T.,
Toojinda T.,
Vanavichit A.,
Tragoonrung S.
Publication year - 2003
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2003.2267
Subject(s) - biology , sterility , oryza sativa , microsatellite , marker assisted selection , genetic marker , population , genotyping , genetics , hybrid , gene , botany , genotype , allele , demography , sociology
The use of thermosensitive genetic male sterility (TGMS) in the development and production of rice ( Oryza sativa L.) hybrids is an alternative to the cytoplasmic‐genetic male sterility (CMS) system. This study aimed to develop TGMS lines with aromatic Thai rice background by molecular marker‐aided breeding. Four microsatellite markers (RM2, RM10, RM11, and RM214) on chromosome 7 in the vicinity of the TGMS gene tms2 and showing polymorphism between the two parents were used in genotyping the mapping population consisting of 157 F 2 plants derived from a cross between Norin PL12 (a TGMS line from Japan) and KDML 105 (a popular aromatic Thai rice cultivar). The RM11 marker was approximately 5 centimorgans (cM) from tms2 while RM2 was approximately 16 cM from it. In this F 2 population, the accuracy of selecting sterile plants with RM2 and RM11 markers was 92 and 97%, respectively. In three backcrosses, the accuracy of selection with markers for either homozygous or heterozygous plants was higher than 90% with RM2. Using RM11, we obtained 89% accuracy for selecting homozygous fertile plants and 59% accuracy for selecting heterozygous plants. The results demonstrated that microsatellite markers were powerful in screening large breeding populations, and these markers facilitated selection for plants possessing the tms2 in an early stage of the crop and without exposing the materials to the required temperature for TGMS gene expression. Three TGMS lines with aromatic Thai rice background were developed and showed complete pollen sterility when maximum temperature was higher than 30°C, 1 to 2 wk after panicle initiation. Up to 77% spikelet fertility was observed when these lines were exposed at temperature below 30°C during the critical stage.