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Morphological Comparison of Progeny Derived from 4 x ‐2 x and 4 x ‐4 x Hybridizations of Lotus glaber Mill. and L. corniculatus L
Author(s) -
Beuselinck P. R.,
Steiner J. J.,
Rim Y. W.
Publication year - 2003
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2003.1741
Subject(s) - biology , lotus corniculatus , germplasm , botany , heterosis , horticulture , hybrid
Unreduced gametes (2 n gametes) from Lotus glaber Mill. (2 n = 2 x = 12) may aid intercrossing with L. corniculatus L. (2 n = 4 x = 24) to produce progeny with a greater heterotic advantage than progeny derived from colchicine‐induced {7‐acetamido‐6,7‐dihydro‐1,2,3,10‐tetramethoxybenzo[a]heptalen‐9(5H)‐one} tetraploid (4 x ) L. glaber The objective of this research was to compare progeny produced from 4 x with 2 x (4 x ‐2 x ) and 4 x with 4 x (4 x ‐4 x ) crosses and determine if 2 n gametes had a heterotic advantage over normal gametes derived from colchicine‐induced tetraploids. Vegetative cuttings of the 2 n ‐gamete‐producing L. glaber genotype 204882‐1 (2 x 204882‐1) were treated with colchicine and successfully yielded an autotetraploid (2 n = 4 x = 24) of 204882‐1 (4 x 204882‐1). Three randomly chosen genotypes from the L. corniculatus germplasm MU‐81 were used as maternal parents in crosses to 2 x 204882‐1 and 4 x 204882‐1. Progeny produced from 4 x ‐2 x crosses were intermediate to or larger than their parents in their leaf and flower morphology. Progeny from 4 x ‐4 x crosses were intermediate to or smaller than their parents in leaf and flower size. Size of leaves and flowers of progeny from 4 x ‐2 x crosses were similar to or larger than 4 x ‐4 x progeny. Morphological comparisons demonstrated that heterotic expression of progeny from 4 x ‐2 x crosses was greater than progeny from 4 x ‐4 x crosses. The results indicate that the generation of interspecific L. corniculatus × L. glaber hybrids via 2 n pollen to be effective, but a means of exploiting any heterotic advantage in these cross‐pollinated species needs to be identified.