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A PCR Assay for Rapid Discrimination of Sterile Cytoplasm Types in Maize
Author(s) -
Liu Zhiyong,
Peter Stefan O.,
Long Muhua,
Weingartner Urs,
Stamp Peter,
Kaeser Olivier
Publication year - 2002
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2002.5660
Subject(s) - biology , cytoplasm , cytoplasmic male sterility , polymerase chain reaction , multiplex , genetics , multiplex polymerase chain reaction , microbiology and biotechnology , gene
A single‐seed, multiplex polymerase chain reaction (PCR) protocol is presented to distinguish the three major types of cytoplasmic male sterility (CMS‐T, ‐C, and ‐S) in maize ( Zea mays L.). Three pairs of PCR primers were designed, corresponding to the chimeric regions of mitochondrial DNA sequences specific for the three types of CMS cytoplasm. The specificity of these primers was tested by means of the inbred line B37 with male‐sterile (T, C, and S) and normal (N) cytoplasm as reference material. Subsequently, a single‐seed PCR assay was developed for the determination of cytoplasm types in a single reaction and applied to a wide range of inbred lines with normal and sterile cytoplasm (T, C, and S) and with predominantly identified subtypes of the major sterile cytoplasm types. Our protocol proved to be a rapid, inexpensive, and reliable procedure for the molecular classification of male sterile maize cytoplasm to the three major categories T, C, and S.