Premium
Genetic and Sequence Analysis of Markers Tightly Linked to the Soybean mosaic virus Resistance Gene, Rsv 3
Author(s) -
Jeong S. C.,
Kristipati S.,
Hayes A. J.,
Maughan P. J.,
Noffsinger S. L.,
Gunduz I.,
Buss G. R.,
Maroof M. A. Saghai
Publication year - 2002
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2002.2650
Subject(s) - biology , soybean mosaic virus , restriction fragment length polymorphism , genetics , gene , amplified fragment length polymorphism , locus (genetics) , polymerase chain reaction , genetic marker , positional cloning , sequence tagged site , virology , gene mapping , virus , plant virus , potyvirus , genetic diversity , population , chromosome , demography , sociology
Soybean mosaic virus (SMV) is a major viral pathogen, affecting soybean [ Glycine max (L.) Merr.] production worldwide. The Rsv 3 gene of soybean confers resistance to three of the most virulent strains (G5–G7) of SMV. The objectives of this study were to map Rsv 3 and develop polymerase chain reaction (PCR) based markers for marker‐assisted selection (MAS) purposes. Disease‐response data were collected from two F 2 mapping populations, L29 ( Rsv 3) × Lee68 ( rsv 3) and Tousan 140 ( Rsv 3) × Lee68 ( rsv 3). Bulk segregant analysis based on amplified fragment length polymorphism (AFLP) markers demonstrated that the Rsv 3 locus maps to the soybean molecular linkage group (MLG) B2 between restriction fragment length polymorphism (RFLP) markers A519 and Mng247. These two tightly linked RFLP markers were converted to PCR‐based markers to expedite MAS. Sequence analysis of the Mng247 genomic region revealed similarity to the consensus sequence of a leucine‐rich repeat (LRR) characteristic of the extracellular LRR class of disease resistance genes. Results from this study will be useful in pyramiding viral resistance genes and in cloning the Rsv 3 gene.