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Genetic Linkage in Soybean
Author(s) -
Mahama A. Assibi,
Lewers Kim S.,
Palmer Reid G.
Publication year - 2002
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2002.1459
Subject(s) - biology , linkage (software) , genetic linkage , glycine , mutant , genetics , chromosome , genetic linkage map , gene , gene mapping , amino acid
Mapping mutants of classical linkage groups 6 and 8 (CLGs 6 and 8) in soybean [ Glycine max (L.) Merr.] can enhance the identification of important agronomic traits. Genetic data suggest that CLGs 6 and 8 may belong to the same linkage group. Our objectives were to determine if CLGs 6 and 8 are the same or different linkage groups and to determine the gene order. Genotypes containing mutants of CLGs 6 and 8 and mutants of other CLGs were crossed in various combinations. Data for the different characters were collected from F 2 populations and F 2:3 families. Recombination values confirmed that CLG 6 characters, Df 2 and Y 11 were linked ( R = 27.0 ± 5.9), Df 2 was linked to Ms 1 (CLG 8) ( R = 24.8 ± 1.2) and to W 1 (CLG 8) ( R = 36.4 ± 1.3), and Y 11 was linked to Ms 1 ( R = 31.7 ± 1.4). F 2 data suggested that Y 11 segregated independently of W 1 , while F 2:3 data indicated the two were linked ( R = 38.4 ± 3.2). Our data indicated that CLGs 6 and 8 belong to the same linkage group, which is molecular linkage group F (MLG F), and chromosome 13. Y 11 and Adh 1 are at the ends of the chromosome segment studied, and Y 23 is located between Ms 6 and St 5 Recombination values among the other loci of CLG 8, and between them and loci of other CLGs were consistent with published values. This information will be useful in the reassignment of CLGs, ordering of loci, and will enhance molecular genetic linkage mapping in soybean.