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Wheat Polyphenol Oxidase
Author(s) -
Demeke Tigst,
Morris Craig F.,
Campbell Kimberly G.,
King Garrison E.,
Anderson James A.,
Chang HakGil
Publication year - 2001
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2001.1750
Subject(s) - quantitative trait locus , biology , population , genetic marker , polyphenol oxidase , genetics , genetic linkage , enzyme , biochemistry , gene , peroxidase , demography , sociology
The enzyme polyphenol oxidase (PPO) has been implicated in discoloration of Asian noodles. The recombinant inbred line (RIL) populations, M6/‘Opata 85’, NY18/CC, and ND2603/‘Butte 86’ were used to investigate the distribution, chromosome location, and number of loci involved in wheat ( Triticum aestivum L.) PPO. PPO activity was measured by means of the substrates L‐DOPA (L‐3,4‐dihydroxyphenyl‐alanine) and L‐tyrosine. The M6/Opata 85 RIL population had a normal distribution, while the ND2603/Butte 86 RIL population had a bimodal distribution for PPO activity (L‐DOPA assay). Transgressive segregants were observed for all three populations. Correlations between L‐DOPA and L‐tyrosine assays for PPO activity were low to medium and could be attributed to substrate specificity and environment. For the combined analysis of M6/Opata 85 RIL populations, the QTL marker Xfba314 (located on chromosome 2D) showed significant association with PPO activity for the L‐DOPA assay. For the combined analysis of NY18/CC, three QTL markers for L‐DOPA, and two different QTL markers for L‐tyrosine, revealed an association with PPO activity at LOD scores of >2.4. The QTL markers for the NY18/CC RIL population were located on chromosomes 2A, 2B, 3D, and 6B. The ND2603/Butte 86 population had relatively few other loci for linkage analysis and only the marker Xbcd907.RV.I located on chromosome 3BS showed a weak association with PPO activity on the basis of the L‐DOPA assay. The identified QTL markers will be useful for marker‐assisted selection as they build upon the evolving maps for these populations, and for resolving in greater detail the genetic basis of PPO activity in wheat.