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Inheritance of Angular Leaf Spot Resistance in Common Bean and Identification of a RAPD Marker Linked to a Resistance Gene
Author(s) -
Ferreira C.F.,
Borém A.,
Carvalho G.A.,
Nietsche S.,
Paula T.J.,
Barros E.G.,
Moreira M.A.
Publication year - 2000
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2000.4041130x
Subject(s) - rapd , biology , phaseolus , leaf spot , cultivar , population , genetics , major gene , gene , primer (cosmetics) , genetic marker , resistance (ecology) , botany , agronomy , chemistry , demography , sociology , genetic diversity , organic chemistry
Angular leaf spot, caused by Phaeoisariopsis griseola (Sacc.) Ferraris, is one of the major diseases affecting the common bean ( Phaseolus vulgaris L.) in Brazil which can lead to severe yield losses. Previous studies demonstrated that cultivar MAR‐2 was resistant to race 63.39 of P. griseola . The objective of this work was to characterize the resistance to angular leaf spot in MAR‐2 in an F 2 population derived from the cross with Ruda (susceptible parent), and also to identify random amplified polymorphic DNA (RAPD) markers linked to the resistance gene. Cultivar MAR‐2 was crossed with Ruda, a “carioca‐type” cultivar susceptible to angular leaf spot, to determine the inheritance of resistance. The results demonstrated that a single dominant gene present in MAR‐2 was responsible for the resistance to P. griseola , race 63.39. Resistant and susceptible DNA bulks from the F 2 population were constructed to identify RAPD markers linked to the resistance gene. Amplification with primer OPE‐04 generated a 500‐bp fragment which distinguished the resistant from the susceptible bulk populations. Co‐segregation analysis of the entire population demonstrated that the RAPD marker was linked to the resistance gene at a distance of 5.8 Cm.