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Progeny Analysis of Glyphosate Selected Transgenic Soybeans Derived from Agrobacterium ‐Mediated Transformation
Author(s) -
Clemente Thomas E.,
LaVallee Bradley J.,
Howe Arlene R.,
ConnerWard Dannette,
Rozman Renee J.,
Hunter Priscilla E.,
Broyles Debra L.,
Kasten Daniel S.,
Hinchee Maud A.
Publication year - 2000
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2000.403797x
Subject(s) - biology , agrobacterium , glyphosate , transformation (genetics) , shoot , genetically modified crops , transgene , explant culture , botany , rhizobiaceae , glycine , gene , genetics , microbiology and biotechnology , bacteria , in vitro , symbiosis , amino acid
Glyphosate selection has been implemented into an Agrobacterium ‐mediated transformation system for soybean [ Glycine max (L.) Merr.]. Cotyledonary explants were excised from 5‐d‐old seedlings and inoculated with an Agrobacterium strain carrying the CP4 gene, which confers tolerance to the herbicide Roundup, and the β‐glucuronidase (GUS) gene. Explants were selected on sublethal levels of glyphosate during shoot initiation and elongation. Glyphosate tolerant shoots were identifiable after 8 to 12 wk of selection. Coexpression of GUS occurred in approximately 80% of the primary transformants (R 0 ). The R 0 plants were fully fertile, with no apparent phenotypic abnormalities. Progeny analysis from 81 independent transgenic soybean events derived from glyphosate selection demonstrated that the majority of the transformants transmitted the transgenes as a single functional locus.