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Inheritance and Allelism Analysis of Hypernodulating Genes in the NOD3‐7 and NOD2‐4 Soybean Mutants
Author(s) -
Vuong T.D.,
Harper J.E.
Publication year - 2000
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2000.403700x
Subject(s) - biology , mutant , genetics , allele , gene , genetic analysis , locus (genetics) , phenotype
Hypernodulating soybean [ Glycine max (L.) Merr.] mutants derived from cv. Williams showed greater early‐season dinitrogen fixation potential and partial tolerance to high levels of NO − 3 Understanding genetic control is essential to manipulation of this trait. Genetic analysis of the NOD1‐3 and NOD4 mutants, and the En6500 mutant, had previously shown that these three mutants were controlled by the same recessive allele at the rj 7 locus. However, genetic control of two additional hypernodulating mutants, NOD3‐7 and NOD2‐4, remained to be verified. We investigated the inheritance and allelic relationship of these latter mutations in relation to those previously studied. The cultivar Harosoy 63 was crossed to the NOD3‐7 and NOD2‐4 mutants to produce F 1 hybrids, F 2 progeny, and F 2 ‐derived families. Phenotypic segregation was examined. For allelism tests, hypernodulating progeny exhibiting purple hypocotyl, which were isolated from the F 2 segregants of the crosses with Harosoy 63, were used for crossing with the NOD2‐4 mutant. Resulting phenotypes were visually evaluated for hypernodulation at 14 d after planting (DAP) in the greenhouse. The results of genetic analysis indicated that a recessive allele was responsible for hypernodulation in the NOD3‐7 and NOD2‐4 mutants. Allelism analysis revealed that although the hypernodulating mutants were isolated from independent mutational events, the rj 7 locus controlled NOD‐type hypernodulating mutants and the En6500 mutant.