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Positioning the wp Flower Color Locus on the Soybean Genome Map
Author(s) -
Hegstad J. M.,
Tarter J. A.,
Vodkin L. O.,
Nickell C. D.
Publication year - 2000
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2000.402534x
Subject(s) - biology , locus (genetics) , genetics , restriction fragment length polymorphism , genetic marker , allele , gene mapping , quantitative trait locus , genotype , glycine soja , molecular marker , population , genetic linkage , cleaved amplified polymorphic sequence , gene , glycine , chromosome , demography , amino acid , sociology
The wp allele generates a pink flowered phenotype in soybean [ Glycine max (L.) Merr.]. Genotypes with W1_wp wp are associated with larger seed size and higher seed protein content. The influence of a single locus on the anthocyanin pathway and seed protein accumulation has not been documented in other species. It is of interest to clone wp by initially identifying linked molecular markers. The objective of this research was to use restriction fragment length polymorphism (RFLP) and simple sequence repeat (SSR) molecular markers to position the wp locus on the soybean public genetic map. Of the 74 markers tested, two RFLP and 14 SSR markers were identified as linked to wp The two RFLP markers linked to wp in this study were previously reported to be associated with quantitative trait loci (QTL) controlling seed protein in a G. soja Siebold & Zucc. × G. max population. It is proposed that wp is located between RFLP locus K011 and SSR locus Satt600. The molecular markers linked to wp were located on linkage group D1b+W of the public soybean genetic map. The knowledge of linked molecular markers will assist in cloning wp , potentially leading to further characterization of how wp influences the anthocyanin pathway and seed protein accumulation.