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Development of PCR‐Based Markers for a High Grain Protein Content Gene from Triticum turgidum ssp. dicoccoides Transferred to Bread Wheat
Author(s) -
Khan I. A.,
Procunier J. D.,
Humphreys D. G.,
Tranquilli G.,
Schlatter A. R.,
MarcucciPoltri S.,
Frohberg R.,
Dubcovsky J.
Publication year - 2000
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2000.402518x
Subject(s) - biology , amplified fragment length polymorphism , triticum turgidum , locus (genetics) , genetics , restriction fragment length polymorphism , quantitative trait locus , allele , genotype , glutenin , genetic marker , cleaved amplified polymorphic sequence , gene , protein subunit , genetic diversity , population , demography , sociology
Grain Protein Content (GPC) of wheat ( Triticum aestivum L. and T. turgidum L.) is important for improved nutritional value and is also one of the major factors affecting breadmaking and pasta quality. A quantitative trait locus (QTL) for high GPC was detected a few years ago in the short arm of chromosome 6B from accession FA15‐3 of Triticum turgidum L. var. dicoccoides New molecular markers are presented here to facilitate the transfer of this high GPC gene into tetraploid and hexaploid wheat cultivars. Two sets of PCR (polymerase chain reaction) primers were designed to amplify regions of the non‐transcribed spacer of the XNor‐B2 locus. This locus was selected because it mapped on the peak of the QTL for GPC. The first pair of allele‐specific primers produced an amplification product only when the T. turgidum var. dicoccoides XNor‐B2 allele was present. The second pair of primers amplified fragment(s) of similar length in the different genotypes that after digestion with the restriction enzyme Bam HI allowed differentiation of the T. turgidum var. dicoccoides allele. Four microsatellites markers were mapped on the short arm of chromosome 6B at both sides of the QTL peak and two on the long arm. Five additional amplified fragment length polymorphism (AFLP) markers were mapped into the QTL region on 6BS. These PCR markers together with 10 restriction fragment length polymorphism (RFLP) markers showed that the hexaploid cultivar Glupro, selected for high GPC, carries a distal segment of chromosome 6BL and a proximal segment of 6BS from dicoccoides accession FA15‐3 encompassing the segment with highest LOD score for the GPC QTL.