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An indirect test using oxalate to determine physiological resistance to white mold in common bean
Author(s) -
Kolkman Judith M.,
Kelly James D.
Publication year - 2000
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci2000.401281x
Subject(s) - sclerotinia sclerotiorum , biology , phaseolus , cultivar , germplasm , wilting , horticulture , agronomy , plant disease resistance , botany , gene , biochemistry
In common bean ( Phaseolus vulgaris L.), the detection of physiological resistance to white mold [ Sclerotinia sclerotiorum (Lib.) de Bary] in the field is confounded by environmental factors and plant avoidance mechanisms. Development of a reliable screening procedure is needed to identify resistant bean germplasm and to develop resistant cultivars. The objective of this study was to determine if oxalate, a primary pathogenicity factor of S. sclerotiorum , could be used to indirectly screen for physiological resistance to white mold in common bean. Cut bean seedlings were placed in a 20 m M oxalate solution in the greenhouse. Genotypes were rated based on differences in wilting response to oxalate. Oxalate ratings of the 27 genotypes were correlated with field ratings of a white mold disease severity index( r=0.58** )and incidence( r=0.57** ), and negatively correlated with yield( r=−0.50** ). The oxalate test is an efficient method to indirectly test for physiological resistance to white mold in common bean.