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Discovery and Characterization of Polymorphic Simple Sequence Repeats (SSRs) in Peanut
Author(s) -
Hopkins M. S.,
Casa A. M.,
Wang T.,
Mitchell S. E.,
Dean R. E.,
Kochert G. D.,
Kresovich S.
Publication year - 1999
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1999.0011183x003900040047x
Subject(s) - biology , primer (cosmetics) , arachis hypogaea , microsatellite , arachis , polymerase chain reaction , genetics , genetic marker , genomic dna , dna profiling , genotype , molecular marker , germplasm , dna sequencing , dna , botany , gene , allele , chemistry , organic chemistry
Cultivated peanut ( Arachis hypogaea L), an important agronomic crop, exhibits a considerable amount of variability for morphological traits and for resistance to diseases and pests. In contrast, molecular marker assays have detected little variation at the nucleic acid level. Identification of molecular markers would be of great help to peanut breeders, geneticists, and taxonomists. The objectives of this work were to identify simple sequence repeat (SSR) markers in cultivated peanut and to test these markers for their ability to discriminate among accessions. Peanut total genomic DNA libraries were constructed and screened with 32 P‐labeled dinucleotide repeats, (GT) 10 and (CT) 10 . DNA sequences were obtained from the SSR‐containing clones and, when possible, primer pairs were designed on the basis of DNA sequences flanking the repeat motif. Primer pairs were tested in polymerase chain reaction (PCR) assays using a collection of 22 peanut DNAs, representing both cultivated peanut and wild species. In all, six SSR markers, five from the library screening procedure and one additional marker obtained from a search of publicly available DNA sequences, detected polymorphisms among the peanut DNAs. Discrimination power was high among the cultivated peanuts, with 17 unique genotypes represented among the 19 accessions tested. From two to 14 DNA fragments were amplified per SSR marker, and as a group, the six markers may amplify up to 10 putive SSR loci. The SSR markers identified in this study were more effective in detecting molecular variation in cultivated peanut than all other DNA‐based markers evaluated to date.