Registration of Four White Mold Resistant Dry Bean Germplasm Lines: 19365‐3,19365‐5,19365‐31, and 92BG‐7

Dry edible bean (Phaseolus vulgaris L.) germplasm lines 19365-3 (Reg. no. GP-183, P1 603035), 19365-5 (Reg. no. GP-184, P1 603036), 19365-31 (Reg. no. GP-185, P1 603037) and 92BG-7 (Reg. no. GP-186, P1 603038) were released by the USDA-ARS in 1997. A major advantage of these lines is their moderate resistance to white mold [caused by Sclerotinia sclerotiorum (Lib.) de Bary], White mold is a major disease problem of dry edible beans grown worldwide, and cultivars grown throughout the United States are susceptible. These four lines also exhibited high yield potential in both tropical (Puerto Rico) and temperate (Michigan, Nebraska, North Dakota, Washington) environments, resistance to bean common mosaic and necrosis viruses (BCMV and BCMNV), and resistance to rust [caused by Uromyces appendiculatus (Pers.) Unger var. appendiculatus; syn. U. phaseoli]. These four lines were evaluated for physiological reaction to white mold in straw tests (1) conducted at the University of Nebraska (2), Cornell University (M.H. Dickson), North Dakota State University, and the USDAARS at Prosser, WA, from 1995 to 1997. Reactions of these four lines to white mold were evaluated in Michigan, Nebraska, and North Dakota in the field from 1995 to 1997 (3). 19365-3 is a small, red dry bean developed from an interspecific Population II (Florida 6-19/Pc-46) using a modified bulk selection of 10 plants for three continuous generations. Population II was cooperatively released in 1985 by the USDA-ARS and the Agricultural Experiment Stations of Puerto Rico and Florida as a heterogeneous Fo bulk possessing different disease resistances, plant habits, and seed sizes, shapes, and colors (4). The common bean parent, Florida 6-19, was an F4 bulk selection for reclining foliage and short internodes from the cross Guatemala 14-2 (Cambridge collection)/'Remus'. The Phaseolus coccineus L. parent, Pc-46, was developed by recurrent selection for multiple disease resistance by N.G. Vakili in Puerto Rico and released by the USDA-ARS in 1979.19365-3 averaged 98 d to harvest maturity at Othello, WA (1997), and exhibited an upright indeterminate Type Ilb-IIIa growth habit (5). Weight of 100 seeds averaged 24 g. In field and greenhouse tests the reaction of 19365-3 to white mold was comparable to 'Bunsi' (known in Canada as 'Ex Rico 23'). Bunsi navy bean has been widely used as a check cultivar because it has some resistance to white mold (2,3,6). 19365-3 exhibited a top necrosis, black root response to BCMNV strains confirming presence of the / gene for resistance to BCMV and BCMNV. This line is resistant to rust pathotypes in Colorado that overcame the Ur-6 rust resistant gene (7) present in 'Olathe' pinto bean but not the Ur-3 gene (7) present in 'Chase' pinto bean. 19365-5 is a small, pink dry bean developed from an interspecific cross (P. vulgaris/P. coccineus/V233B) using a modified bulk selection of 10 plants, from the F5 to F7 generations. The original P.vulgaris parent was a dry bean derived by recurrent selection for seed yield and resistance to diseases endemic to Puerto Rico. The P. coccineus parent was developed by recurrent selection for seed yield and multiple disease resistance from materials released by N.G. Vakili (USDA-ARS) in Puerto Rico in 1979. The backcross parent, 233B, is an advanced dry bean developed by G.F. Freytag (USDA-ARS) in Puerto Rico with high yield and semiupright architecture. 19365-5 averaged 99 d to harvest maturity and exhibited an indeterminate Type Ill-Ilia growth habit. Weight of 100 seeds averaged 31 g. 19365-5 is comparable to Bunsi for field and greenhouse resistance reaction to white mold. 19365-5 has the / gene, and segregates for immune resistance to rust. 19365-31 is a black dry bean developed from an interspecific Population I ('Sprite'/Pc-46) using a modified bulk selection of 10 plants for three continuous generations. Population I (4) was developed and released as described above for Population II. Sprite is a garden-shipper market snap bean. 19365-31 averaged 98 d to harvest maturity and exhibited an indeterminate Type III prostrate growth habit. Weight of 100 seeds averaged 22 g. In some field and greenhouse tests, 19365-31 outperformed Bunsi for resistance to white mold. 19365-31 segregated for the / gene and expressed immunity to rust. 92BG-7 is a black bean developed from a single-plant selection from the interspecific triple cross KH ('Panache'/4/'WulmaV/ 'Colorado'/P. coccineus subsp.purpurascens Freytag /3/P. lunatus L./P. coccineus). KH was released in 1985 by the USDA-ARS in Mayagiiez, PR, and by INRA, Versailles, France, as an advancedgeneration, heterogeneous bulk possessing different disease resistances, plant habits, and seed sizes, shapes, and colors. Panache, Wulma, and Colorado are common bean cultivars (P. vulgaris). 92BG-7 averaged 91 d to harvest maturity, and exhibited an indeterminate semiupright Type Ilia growth habit. Weight of 100 seeds averaged 22 g. In some field and greenhouse tests, 92BG-7 expressed greater resistance to white mold than Bunsi. 92BG-7 possesses the / gene and immune resistance to rust. These four lines will be most useful for transferring resistance to white mold into dry edible bean market classes with small to medium-sized seed. Limited quantities of seed are available from the corresponding author. We ask that appropriate recognition of source be given when this germplasm contributes to a new cultivar.