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DNA‐Based Markers for a Recessive Gene Conferring Anthracnose Resistance in Sorghum
Author(s) -
Boora Khazan S.,
Frederiksen Richard,
Magill Clint
Publication year - 1998
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1998.0011183x003800060048x
Subject(s) - biology , rapd , bulked segregant analysis , genetics , sorghum , allele , cultivar , genetic marker , gene , dna , botany , gene mapping , genetic diversity , agronomy , chromosome , population , demography , sociology
Resistance to anthracnose in sorghum [ Sorghum bicolor (L.) Moench] accession SC326‐6 was found to segregate as a simple recessive trait in a cross to the susceptible cultivar BTx623.F3 progeny tests following self pollination of 115 F 2 individuals identified homozygous resistant and susceptible F 2 plants for use in bulked‐segregant analysis. DNA from the parental cultivars and the bulks was screened by PCR amplification with 300 RAPD (random amplified polymorphic DNA) primers. Two RAPD primers amplified a sequence that co‐segregated with the recessive resistance allele, while another amplified a band linked to the susceptible allele.