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Molecular Markers Associated with T and Z , Two Genes Controlling Partly Colored Seed Coat Patterns in Common Bean
Author(s) -
Brady L.,
Basset M. J.,
McClean P. E.
Publication year - 1998
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1998.0011183x003800040031x
Subject(s) - biology , rapd , genetics , gene , molecular marker , genetic marker , centimorgan , coat , phaseolus , allele , gene mapping , botany , genetic diversity , population , paleontology , demography , sociology , chromosome
Five genes control the expression of partly colored seed coat patterns in common bean ( Phaseolus vulgaris L.), viz., T , Z , L , Bip , and J. Because these genes have complex interactions, giving various seed coat patterns, it is often difficult to assign specific effects to a particular gene. The problem is compounded by multiple alleles at T , Z , and L . Molecular markers linked to those pattern genes have the potential to assist in identifying the genes involved in specific interactions. We have begun a series of experiments to identify useful molecular markers linked to these genes. A group of three parental genotypes and five backcross lines were screened by random amplified polymorphic DNA (RAPD) techniques to identify putative markers. Potential markers were then mapped in appropriate BC 3 ‐F 2 populations. To date, recombination has not been detected between RAPD markers OAM13 1350 , and OMI9 400 and the T gene. A single recombinant was detected between RAPD marker OAMl0 560 and the Z gene, and the marker mapped at a distance of 1.4 centimorgans (cM).