Premium
Rhizomatous Lotus corniculatus L.: III. Introgression of Rhizomes into Autogamous Germplasm
Author(s) -
Nualsri C.,
Beuselinck P. R.,
Steiner J. J.
Publication year - 1998
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1998.0011183x003800020038x
Subject(s) - biology , germplasm , lotus corniculatus , hybrid , rhizome , introgression , botany , polyploid , horticulture , genetics , ploidy , gene
of genes for rhizomes from wild Lotus corniculatus L. germplasm into domesticated germplasm may be an important step towards improving persistence of cultivated L. corniculatus , normally a cross‐pollinated herbage crop. This study was initiated to determine if rhizomes could be transferred to a L. corniculatus germplasm that sexually reproduces via autogamy. Autogamy is an unusual reproductive expression in this typically self‐incompatible species. Compatibility was high between the autogamous germplasm AG‐S4 and five wild Moroccan accessions with rhizomes. Higher crossing success was obtained when AG‐S4 was used as a maternal parent. Most seeds produced were normal in appearance and size and had high germination. The resulting F 1 progeny were morphologically intermediate between the parents for size and shape of leaf and floral characters, but appeared more similar to their Moroccan parents. Differences between reciprocal crosses were not evident. Hybridity of progeny was confirmed by random amplified polymorphic DNA (RAPD) analysis using eight primers. Fertility of F 1 progeny was low relative to their parents. Meiosis of parental genotypes was characterized by a high frequency of bivalents, but the meiotic behavior of their F 1 hybrids was more irregular. The frequency of univalents varied from 0 to 8 with an average 2.4 per ceil, indicating homology of chromosomes between AG‐S4 and Moroccan accessions was not complete. Rhizomes were successfully expressed in F 1 hybrids and a small number were found to also be autogamous. The autogamous hybrids will be important tools to elucidate the inheritance of rhizomes in L. corniculatus . Selection among the hybrids could lead to autogamous, rhizomatous cultivars of L. corniculatus .