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Chemical Characterization of a Shriveled Seed Trait in Peanut
Author(s) -
Jakkula Lakshmi R.,
O'Keefe Sean F.,
Knauft David A.,
Boote Kenneth J.
Publication year - 1997
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1997.0011183x003700050025x
Subject(s) - biology , arachis hypogaea , storage protein , cultivar , sucrose , crop , rapeseed , sativum , botany , agronomy , food science , gene , biochemistry
Seed morphology mutations affecting the major seed components in pea ( Pisum sativum L.) and maize ( Zea mays L.) opened specialty markets for these crops. Peanut ( Arachis hypogaea L.) is an important oil and food crop of the world. Genetic alteration of seed composition may be useful in expanding markets for this crop. Some lines from the University of Florida peanut breeding program showed seed shriveling characteristic even when harvested mature. In the present study, three shriveled lines (529B, 563A, and 647A) were chemically characterized and compared with a normal‐seeded cultivar, Sunrunner. They were found to have only one third to two thirds the amount of storage lipid and double the amount of sucrose as normal peanuts. Differences were also observed for seed protein concentration, when expressed on a defatted meal basis. Higher relative proportions of phospholipid to triacylglycerol may indicate a block in the biochemical pathway for the conversion of sucrose into triacylglycerol in the shriveled seed mutants. Sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) patterns revealed that there were differences in the levels of various proteins between normal seed and shriveled seed phenotypes. Differences were identified in the level of accumulation of storage lipid and also in the various proteins during the development of the shriveled seed.