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Cuphea viscosissima Mutants with Decreased Capric Acid
Author(s) -
Knapp S. J.,
Crane J. M.,
Tagliani L. A.,
Slabaugh M. B.
Publication year - 1997
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1997.0011183x003700020007x
Subject(s) - capric acid , biology , locus (genetics) , allele , genetics , fatty acid , lauric acid , gene , biochemistry
The range of medium‐chain oils produced by Cuphea viscosissima Jacq., a naturally rich source of capric acid (10:0), can be broadened by developing lines with increased caprylic (8:0), lauric (12:0), myristic (14:0) acid. Our objectives were to (i) describe the inheritance of a series of mutants with decreased 10:0 (CPR‐1, CPR‐2, CPR‐4, CPR‐5, and CLM‐1) and (ii) develop lines with fatty acid compositions different from other mutant lines. CPR‐1, CPR‐2, and CPR‐5 had ~310 g kg ‐1 less 10:0 than the wild type (WT), whereas CPR‐4 had 62 g kg ‐1 less 10:0 and CLM‐1 had 50 g kg ‐1 less 10:0 than the wild type. We assayed the fatty acids of 100 progeny from 10 F 2 populations segregating for one or more mutations. CPR‐1, CPR‐2, and CPR‐5 produced three non‐overlapping capric acid classes and segregated as single incompletely dominant mutations. CPR‐1 × CPR‐2 and CPR‐1 × CPR‐5 F 2 progeny had continuous 8:0 and 10:0 distributions. The CPR‐1 × CPR‐5 cross produced several F 2 progeny with atypical 8:0 and 10:0 phenotypes, while the CPR‐1 × CPR‐2 cross produced no F 2 progeny different from either parent. CPR‐1 and CPR‐2 seem to be allelic mutations affecting the cpr locus. CPR‐5 could be an allele of the cpr locus or of a locus tightly linked to cpr . CPR‐4 × WT and CLM‐1 × WT F 2 progeny had continuous 10:0 distributions. CPR‐1, CPR‐4, and CLM‐1 seem to be non‐allelic mutations. VS‐320, an F 3 line developed from the CPR‐1 × CLM‐1 F 2 population, had 334 g kg ‐1 8:0 and 396 g kg ‐1 10:0. VS‐321, an F 3 line developed from the CPR‐4 × CPR‐5 F 2 population, had 247 g kg ‐1 8:0 and 361 g kg ‐1 10:0. The 8:0 or 10:0 contents of these lines are lower or higher than previously reported for C. viscosissima .