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Micropropagation of Switchgrass by Node Culture
Author(s) -
Alexandrova K. S.,
Denchev P. D.,
Conger B. V.
Publication year - 1996
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1996.0011183x003600060049x
Subject(s) - micropropagation , panicum virgatum , biology , shoot , botany , murashige and skoog medium , growth regulator , tissue culture , horticulture , somaclonal variation , microbiology and biotechnology , bioenergy , biofuel , in vitro , biochemistry
Switchgrass ( Panicum virgatum L.) is naturally outcrossing; therefore, maintenance of genotype is difficult through sexual propagation. The objective of this study was to develop a micropropagation procedure for the multiplication of desired or selected genotypes. Nodal segments were surface sterilized, split longitudinally, and placed with the cut surface on solid MS medium containing 30 g L −1 maltose and different concentrations of 6‐benzylaminopurine (BAP) as the only growth regulator. Best shoot proliferation was obtained with 12.5 μ M BAP and culture at 29°C. Shoots were easily rooted on MS medium without BAP. Under optimum conditions, it is possible to produce approximately 500 plantlets from one parent plant in 12 wk.