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Immunoflourescent Localization of RuBPCase in Degraded C 4 Grass Tissue
Author(s) -
Miller M. S.,
Moser L. E.,
Waller S. S.,
Kirch B. H.,
Klopfenstein T. J.
Publication year - 1996
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1996.0011183x003600010030x
Subject(s) - panicum virgatum , panicum , biology , rumen , vascular bundle , digestion (alchemy) , incubation , parenchyma , forage , poaceae , protein degradation , zoology , biochemistry , agronomy , botany , chemistry , fermentation , chromatography , microbiology and biotechnology , bioenergy , biofuel
Digestion‐resistant tissues found in C 4 grasses may allow soluble protein to escape rumen degradation. The objective of this study was to use immunofluorescent localization to follow loss of ribulose 1,5‐bisphosphate carboxylase/oxygenase (RuBPCase) from switchgrass ( Panicum virgatum L.) and big bluestem ( Andropogon gerardii Vitman) parenchyma bundle sheath cells (BSC) during rumen degradation. Fluorescent signal was observed from switchgrass and big bluestem BSC through 24 and 16 h in situ digestion, respectively, and from BSC associated with both intact tissue fragments, and isolated vascular bundles in omasal digesta and fecal material from steers ( Bos tarus L.) grazing switchgrass. Immunofluorescent localization demonstrated that in certain C 4 grasses (i) parenchyma BSC can protect RuBPCase from degradation through 24‐h in situ incubation, (ii) BSC containing RuBPCase can exit the rumen prior to degradation, and (iii) protein protected by BSC can escape degradation in the whole gastrointestinal tract and be excreted.