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Agronomic Performance of Barley Lines Derived from Tissue Culture
Author(s) -
Bregitzer Phil,
Poulson Malyn
Publication year - 1995
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1995.0011183x003500040038x
Subject(s) - biology , cultivar , hordeum vulgare , tissue culture , callus , somaclonal variation , poaceae , selection (genetic algorithm) , plant breeding , botany , agronomy , microbiology and biotechnology , horticulture , in vitro , genetics , artificial intelligence , computer science
The practical application of in vitro genetic manipulations to plant breeding may be hindered by unselected and potentially undesirable somacional variation. To determine the potential for recovering cultivars from tissue cultures without somaclonai variation, 30 families of tissue culture derived lines from six barley ( Hordeum vulgare L.) cultivars were analyzed for agronomic performance. Each family was derived from a single regenerated plant that in turn was derived from an immature embryo callus culture. Four tosix R 2 plants were selected from each family based on phenotypic similarity to the uncultured parental controls. One‐hundred‐seventy‐four R 2 derived lines were advanced by bulking to the R 5 generation. The results of replicated field trials indicated that the agronomic performance for the majority of these lines was altered by passage through tissue culture and that the alterations were generally undesirable. Cultivar‐dependent differences were noted in the frequency and degree of agronomic alterations. Families within cultivars was an important source of variation, but selections within families was not. Successful selection of tissue culture derived barley lines without somocional variation therefore may be cultivar dependent and may require screening large populations of regenerated plants.