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Production and Identification of Chromosome Specific RAPD Markers for Langdon Durum Wheat Disomic Substitution Lines
Author(s) -
Wang Richard RC.,
Chen Jianli,
Joppa Leonard R.
Publication year - 1995
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1995.0011183x003500030042x
Subject(s) - rapd , biology , chromosome , genetics , genome , gene , population , demography , sociology , genetic diversity
Random amplified polymorphic DNA (RAPD) analyses were performed with DNA extracted from a complete set of Langdon durum wheat ( Triticum turgidum L. var. durum ) disomic substitution lines and their parents (Langdon and ‘Chinese Spring’) with the objective of assigning chromosomal locations for RAPD markers. Seven out of 12 arbitrary decamer primers yielded 11 products that could be assigned to a specific chromosome of the A‐ or B‐genome from Langdon durum or to a D‐genome chromosome of Chinese Spring. The 11 RAPD markers were assigned to three A‐genome, five B‐genome, and one D‐genome chromosome. Chinese Spring ditelosomic lines were used to assign four RAPD markets to specific chromosome arms. Most of the markers were present in both Chinese Spring and Langdon, but one was genotype specific (e.g., J11c was present on Langdon chromosome 1B only). These reproducible RAPD markers of specific wheat chromosomes are additional tools for genome mapping.