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RFLP and Cytogenetic Analyses of Hybrids between Festuca mairei and Lolium perenne
Author(s) -
Chen C.,
Sleper D. A.,
West C. P.
Publication year - 1995
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1995.0011183x003500030012x
Subject(s) - biology , hybrid , lolium perenne , genome , festuca , ploidy , botany , restriction fragment length polymorphism , lolium , genetics , poaceae , genotype , gene
Information is lacking on the genomic relationships between Festuca mairei St. Yves and Lolium perenne L. Tetraploid (2 n = 4 x = 28, where x = the related affinity of the more closely related genome) F. mairei , diploid (2 n = 2 x = 14) L. perenne , and their intergeneric hybrids were used to determine their genomic relationships based on RFLP patterns of parental species and meiotic chromosome pairing of the hybrids. Among 41 heterologous probes randomly chosen from a F. arundinacea Pst I‐genomic library, 70 and 87% hybridized to L. perenne and F. mairei , respectively. However, different restriction patterns occurred between the two species, indicating their distant relationship. Eighteen triploid (3x) and two tetraploid hybrids between F. mairei and L. perenne were obtained. The formation of 4 x hybrids from 4 x × 2 x crosses confirmed that L. perenne is a 2 n pollen producer. Triploid and tetraploid hybrids had high levels of chromosome pairing with an average of 6.60 and 11.62 bivalents, respectively. Optimization analysis of meioses in 3 x and 4 x hybrids strongly confirmed that F. mairei is a fragmented allotetraploid with MMM′M′ genomes. The M genome is closely related to the M′ genome. The L genome of L. perenne is, however, distantly related to the M and M′ genomes.