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Rapid, Inexpensive Method for Determining Glucose Concentrations in Cotton Bolls and Other Plant Tissues
Author(s) -
Timpa Judy D.,
Striegel Andre M.,
Abellanosa Alan L.,
Triplett Barbara A.
Publication year - 1995
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1995.0011183x003500010051x
Subject(s) - lint , malvaceae , gossypium hirsutum , gossypium , reagent , glucose oxidase , biology , fiber crop , serum glucose , chromatography , biochemistry , microbiology and biotechnology , botany , agronomy , chemistry , organic chemistry , enzyme , insulin
Our objective was to develop a rapid, specific, and inexpensive method for measuringg lucose concentrations in bolls of cotton ( Gossypium hirsutum L.). A new procedure was developed, based on a reagent strip for measuring blood glucose in diabetic patients, and compared with three other glucose methods by assaying glucose standard solutions. The strip results were significantly correlated ( r = 0.948) with known concentrations of glucose and comparable to other methods in both precision and accuracy. For aqueous extracts of locules (lint and seed) from 15 days‐post‐anthesis (DPA) cotton bolls, glucose levels were −10 mg g −1 fresh weight. The reagent strip strongly correlated to glucose hexokinase, glucose oxidase, and anthrone methods with correlation coefficients ( r ) of 0.983, 0.994, and 0.974, respectively. Thus, the strip technique should be useful for routine glucose determinations in cotton bolls considering the time (−2 rain/sample), cost (−.70/sample), and portability.