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Transgressive Segregation for Palmitic Acid in Seed Oil of Soybean
Author(s) -
Wilcox James R.,
Burton Joseph W.,
Rebetzke Gregory J.,
Wilson Richard F.
Publication year - 1994
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1994.0011183x003400050020x
Subject(s) - palmitic acid , biology , locus (genetics) , transgressive segregation , allele , fatty acid , genetics , biochemistry , gene , quantitative trait locus
Palmitic acid is the major saturated fatty acid in soybean [ Glycine max (L.) Merr.] oil. Reducing levels of palmitic acid should enhance the nutritive value of soybean oil for human consumption. The low palmitic acid strain C1726 (81 g kg −1 16:0), carrying the fap1 allele, was crossed with two strains developed independently of C1726, N79‐ 2077‐12 and N90‐2013, both with about 60 g kg −1 16:0. Progenies from these crosses were evaluated to determine genetic relationships controlling palmitic acid in these two groups of strains. Palmitic acid concentrations in the oil of F 2 seeds, F 3 seeds on F 2 plants, and seeds from F 3 families were determined by gas chromatography. Transgressive segregates averaging 44 and 96 g kg −1 16:0 were identified in the segregating populations. Both F 2 plants and F 3 families were identified that were either homozygous or heterozygous for loci controlling palmitic acid. Chi‐square analyses for a 1 homozygous P1 type/1 homozygous P2 type/1 homozygous recombinant type (high 16: 0)/1 homozygous recombinant type (low 16:0)/12 plants or families heterozygous at either locus demonstrated that two loci controlling levels of 16:0 were segregating in these populations. A correlation coefficient of 0.97 calculated between palmitic acid values of homozygous F 2 plants and their F 3 family means demonstrated that the homozygous classes were true breeding for palmitic acid. The locus in C1726 was fapl ; the other locus, in N79‐2077‐12 and N90‐2013, has not been designated. These populations provide additional sources of low palmitic acid to enhance the nutritional value of soybean oil.