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Russian Wheat Aphid Resistance in Barley: Inheritance and Linked Molecular Markers
Author(s) -
NietoLopez Rosa M.,
Blake Thomas K.
Publication year - 1994
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1994.0011183x003400030010x
Subject(s) - biology , russian wheat aphid , introgression , hordeum vulgare , backcrossing , germplasm , cultivar , molecular marker , chlorosis , genetic marker , genetics , aphid , plant disease resistance , gene , pest analysis , botany , aphididae , poaceae , homoptera
Russian wheat aphid (RWA), Diuraphis noxia (Mordvilko), is an important pest of small grains cereals in many areas throughout the world. This research was conducted to determine the inheritance of resistance and to identify molecular markers of resistance in the barley ( Hordeum vulgare L.) lines PI366444 and PI366453. Artificial infestation was performed in the field and growth chamber. Sodium dodecyl sulphate‐polyacrylamide gel electrophoresis, Southern blotting, and polymerase chain reaction (PCR) techniques were used to determine plant genotypes. The PI366444 and PI366453 lines were intercrossed and each was also crossed with the susceptible cultivars Stark and Bearpaw. Allelism tests of F 2 progeny showed that the resistant lines shared common or tightly linked resistance genes. Segregation ratios from crosses among resistant and susceptible plants, measured using F 3 families, indicated that there were at least two resistance genes in both of the P1 lines. Two different regions in the barley genome were associated with RWA resistance genes. Variation for both leaf chlorosis and leaf rolling in F 2 plants from crosses with both resistant lines was associated with the sequence‐tagged‐site (STS) PCR markers B‐hordein and D14 on the short arm of Chromosome 5. The STS‐PCR marker ABG8 on Chromosome 2 was associated with leaf rolling in one cross. Barley breeding programs throughout North America have devoted significant time and resources to backcrossing RWA resistance genes into acceptable cultivars. The molecular markers described in this report may assist barley breeding programs in introgression and fixation of linked resistance genes into useful germplasm.