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Detection of Ergoline Alkaloids in Endophyte‐Infected Tall Fescue by Immunoassay
Author(s) -
Hill N. S.,
Agee C. S.
Publication year - 1994
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1994.0011183x003400020041x
Subject(s) - immunoassay , biology , chromatography , alkaloid , monoclonal antibody , high performance liquid chromatography , extraction (chemistry) , lysergic acid , festuca arundinacea , botany , antibody , biochemistry , poaceae , chemistry , immunology
Previously, a monoclonal antibody was developed to the lysergic ring common to ergoline alkaloids (EA). The objectives were to develop an immunoassay for EA in tall fescue by determining (i) time requirement; (ii) tissue mass, and (iii) optimum volume of extraction solution necessary for detection protocols with repeatable results; and (iv) the acceptability of oven‐ versus freeze‐dried samples. A competitive enzyme‐linked immunosorbent assay (ELISA) was conducted on plant extracts (0‐548 μg kg −1 ergovaline). Regressions of ELISA and high performance liquid chromatography (HPLC) data gave good fits ( R 2 ≥ 0.87, MSE ≤ 0.03). Using 0.10 g (±0.01 g) of oven‐dried tissue, and extraction for 15 min in a 1:40 dilution, we analyze 200 samples daily.