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Backcross (BC2‐BC4)‐Derived Fertile Plants from Glycine max and G. tomentella Intersubgeneric Hybrids
Author(s) -
Singh R. J.,
Kollipara K. P.,
Hymowitz T.
Publication year - 1993
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1993.0011183x003300050026x
Subject(s) - biology , backcrossing , hybrid , botany , rootstock , ploidy , perennial plant , glycine soja , gamete , locus (genetics) , sterility , horticulture , genetics , glycine , gene , sperm , amino acid
Wild perennial relatives of the soybean [ Glycine max (L.) Merr.] have not been utilized to broaden the genetic base of the crop. This study provides information on production, morphology, cytology, and breeding behavior of backcross (BC 2 to BC 4 )‐derived fertile plants from soybean (2 n = 40, genome GG) and G. tomentella Hayata (2 n = 78, genome DDEE). The main hurdle was to obtain BC~ plants ( G. max cv. Altona) × ( G. tomentella , Pl 483218)] → F 1 , 2 n = 59, genome GDE → colchicine treatment (CT) → 2 n = 118, GGDDEE × soybean cv. Clark 63 → BC 1 , 2, n = 76 (expected 2 n = 79). Three sterile BC 2 plants (2 n = 58, 2 n = 56, 2 n = 55) were produced. Procedures for immature seed‐rescue were utilized to obtain F, amphiploid, BC 1 and BC 2 plants. Soybean cv. Clark 63 was used as the recurrent parent in the backcrossing programs. The range of chromosome number among BC 3 plants was 2 n = 41 to 2 n = 52, and among BC 4 plants was 2 n = 40 to 64. A hypertriploid (2 n = 64) in BC 4 plant originated after fertilization of an unreduced egg ( n = 44) by a normal haploid ( n = 20) male gamete. Phenotypes of BC 3 and BC 4 plants were dissimilar but generally resembled closely to the soybean cv. Clark 63. We expect to isolate a series of aneuploid lines from BC 3 to BC 6 generations that may open up the path to introgress desired traits from wild perennial Glycine species to the cultivated soybean.