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Genotypic Comparison of Heat Shock Protein Synthesis in Sorghum
Author(s) -
Jorgensen Julie A.,
Rosenow Darrell T.,
Nguyen Henry T.
Publication year - 1993
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1993.0011183x003300030042x
Subject(s) - sorghum , biology , heat shock protein , intraspecific competition , genetic variation , genotype , gel electrophoresis , heat stress , seedling , genetic variability , botany , genetics , gene , agronomy , zoology , ecology
High temperature stress is an environmental factor limiting sorghum ( Sorghum bicolor (L.) Moench) productivity. We determined the optimum heat‐shock protein (HSP) induction temperature in sorghum leaves and investigated intraspecific genetic variability in HSP synthesis among inbred lines RTx430, SC599‐6, and SC326‐6. One dimensional electrophoresis indicated an optimum 42 °C synthesis temperature in seedling leaves. Analysis of genetic variation by two‐dimensional electrophoresis showed only three genotype‐specific but many common HSPs. Sixteen of the common HSPs were synthesized in control samples; however, synthesis during high temperatures was dramatically enhanced. The results suggest limited variation in HSP synthesis and predominant constitutive HSP synthesis among these sorghum lines.