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Selection of Cultured Wheat Cells for Tolerance to High Temperature Stress
Author(s) -
Wang Wen Chung,
Shang Xiao Min,
Yücel Meral,
Nguyen Henry T.
Publication year - 1993
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1993.0011183x003300020020x
Subject(s) - biology , cell culture , heat shock , cell , in vitro , microbiology and biotechnology , heat shock protein , biochemistry , genetics , gene
In vitro culture offers approaches to study cellular responses and to obtain cells tolerant to high temperature stress. The objectives of this study were to select wheat cells that tolerate high temperature stress, study cell line stability and cytogenetic characteristics, and compare protein synthesis in selected and control cell lines after exposure to heat stress. Three of 600 colonies plated were recovered after cells had been exposed to 48 °C. Forty‐five percent of the cells of the tolerant cell line, M 48 , survived when they were returned to a normal temperature (22 °C) for mo and rechallenged with a 48 °C treatment. The control cell line exhibited 0.8% survival under this treatment. Chromosomal elimination and genomic rearrangements were observed extensively for the cell lines before and after heat selection. Heat selection did enhance the occurrence of polytene chromosomes and the frequency of extrachromosomal DNA. When cell viability was evaluated in cell suspension cultures, M 48 had a higher percentage of viable cells than the control cell line. Analysis of 35 S‐methionine in vivo labeled proteins showed that the selected M 48 cell line maintained the synthesis of most normal proteins under a heat shock of 40 °C for 4 h, with synthesis of several unique low molecular weight heat‐shock proteins (HSPs). quantitative in crease in high molecular weight HSPs was also observed in this cell line. This study demonstrated that a heat‐tolerant cell line of wheat can be obtained from in vitro selection, and that this phenomenon was associated with the synthesis of specific proteins under heat stress.