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Duplications Complicate Genetic Mapping of Rf4, a Restorer Gene for cms‐C Cytoplasmic Male Sterility in Corn
Author(s) -
Sisco Paul H.
Publication year - 1991
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1991.0011183x003100050036x
Subject(s) - cytoplasmic male sterility , biology , genetics , centimorgan , locus (genetics) , restriction fragment length polymorphism , gene , sterility , chromosome , zea mays , inbred strain , gene mapping , genotype , agronomy
Previous studies of the genetics of fertility restoration for C‐type cytoplasmic male sterility (cms‐C) in corn ( Zea mays L.) have produced conflicting results. The objective of this study was to localize the restorer gene(s) for cms‐C using restriction fragment length polymorphism (RFLP) analysis. Rf4 , a single dominant restorer gene for cms‐C found in inbred A619, was localized to Chromosome 8, ≈2 centimorgans from an RFLP marker locus, NPI114A . The presence in corn of extensive nucleotide duplication complicated the analysis. Because the region of Chromosome 8 near Rf4 is duplicated on Chromosome 3, an additional restorer gene for cms‐C may eventually be found on Chromosome 3.