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Cross‐Pollination Technique for Spontaneously Self‐Pollinated Sweetclover
Author(s) -
Miller J. E.,
Viands D. R.,
LaRue T. A.,
Gorz H. J.
Publication year - 1991
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1991.0011183x003100020050x
Subject(s) - biology , hybrid , accession , pollination , botany , inheritance (genetic algorithm) , genetics , gene , pollen , european union , business , economic policy
There is increased interest in using a dwarf, spontaneously self‐pollinated accession (U389) of annual, white‐flowered sweetclover ( Melilotus alba Desr.) for studying N 2 fixation and for characterizing the photosynthetic apparatus. Genetic analysis of this accession may therefore be required. In a study of the inheritance of 10 nonnodulating U389 mutants > 2000 crosses made in the late bud stage were unsuccessful probably because florets were mishandled during the crossing procedure. Development of an improved crossing technique resulted in production of hybrid seed from 20% of the emasculated and pollinated florets. This successful technique is described in detail for future use by researchers making crosses with spontaneously self‐pollinated sweetclovers similar to U389.