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Identification of Four Gossypium sturtianum Monosomic Alien Addition Derivatives from a Backcrossing Program with G. hirsutum
Author(s) -
Rooney William L.,
Stelly David M.,
Altman David W.
Publication year - 1991
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1991.0011183x003100020024x
Subject(s) - biology , backcrossing , genetics , chiasma , meiosis , gossypium , genome , introgression , chromosome , botany , japonica , karyotype , indel , gene , genotype , single nucleotide polymorphism
Alien chromosome addition lines provide means to distinguish effects of specific alien chromosomes, to detect homoeologies between chromosomes of different species, and to conduct chromosome‐specific introgression. Over the last few years, numerous monosomic addition (MA) stocks were derived from interspecific backcrosses of Gossypium sturtianum J.H. Willis (2 n = 2 x = 26, C 1 genome) with G. hirsutum L. [2 n = 4 x = 52, (AD), genome] as recurrent parent. Using 10 MA plants of varied pedigrees from this project, our objectives were to (i) identify and characterize different MA stocks, (ii) determine the phenol) pic effect of each addition chromosome, and (iii) estimate the frequency of (AD) 1 ‐C 1 recombination. We identified 4 distinct G. sturtianum MA types among the 10 analyzed, and have temporarily designated these as C 1 ‐A, C 1 ‐B, C 1 ‐C, and C 1 ‐D. The C 1 ‐A, C 1 ‐B, and C 1 ‐D MA stocks differed phenotypically from the recurrent parent, while the C 1 ‐C MA stock was phenotypically indistinguishable from the recurrent parent. None of the MA chromosomes was pollen transmissible, but all were ovule transmissible in vivo. Frequencies of ovule transmission from the MA chromosomes ranged 13.7% for C 1 ‐B to 77% for C 1 ‐A. Cytogenetic analyses of the parental stocks and their backcross progenies revealed low but nonetheless increased frequencies of abnormal meiotic chromosomal configurations, possibly due to previous intergenomic recombination, the meiotic disturbances, or both. Genetic data to substantiate recombination have not yet been obtained, but chiasmata were observed at a frequency of 1.7% per meiotic C 1 chromosome in the MA stocks, indicating that recombination with chromosomes of G. hirsutum is occurring, albeit infrequently. The establishment and characterization of these four MA stocks will facilitate development of dispersed repetitive genomic probes for the C 1 genome, analysis of interspecific C 1 ‐to‐(AD) 1 genomic introgression, and the derivation of additional C 1 MA stocks.