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Starch Gel Electrophoresis Used for Identification of Turf‐type Cynodon Genotypes
Author(s) -
Vermeulen P. H.,
Beard J. B.,
Hussey M. A.,
Green R. L.
Publication year - 1991
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1991.0011183x003100010049x
Subject(s) - biology , cynodon , phosphoglucomutase , starch , cynodon dactylon , genotype , botany , malate dehydrogenase , aconitase , horticulture , food science , biochemistry , enzyme , gene
A reliable technique is needed to facilitate the identification of cultivars of turf‐type Cynodon species because of difficulties encountered using morphological traits alone. This study was conducted to determine the potential for using starch gel electrophoresis to identify 21 Cynodon genotypes. Six enzyme systems (aconitase, alcohol dehydrogenase, phosphoglucomutase, phosphoglucoisomerase, shikimic acid dehydrogenase, and triosephosphate isomerase) were used because of ease of resolution. Plant material was grown in a greenhouse or growth chamber and crude protein extracts were made from recently emerged leaf blades. Examination of banding patterns allowed identification of 16 unique genotypes (‘A‐22’, ‘A‐29’, ‘Bayshore’, ‘Everglades’, ‘FB‐119’, ‘Midiron’, ‘Midway’, ‘Ormond’, ‘Santa Ana’, ‘Sunturf’, ‘Tiffine’, ‘Tifgreen II’, ‘Tiflawn’, ‘Tifway’, ‘Tifway II’, and ‘Vamont’) using three of the enzyme systems (aconitase, phosphoglucomutase, and phosphoglucoisomerase). The five remaining genotypes were separated into two distinct groups: (i) ‘Pee Dee’, ‘Tifgreen’, and ‘Tifdwarf’ and (ii) ‘Texturf 10’ and ‘Texturf if’. It was concluded from this study that starch gel electrophoresis is useful in genotypic identification with Cynodon species. Additional isoenzyme characterizations are required to achieve individual identification of all genotypes in the collection.