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Heat‐Shock Protein Synthesis and Accumulation in Diploid Wheat
Author(s) -
Vierling Richard A.,
Nguyen Henry T.
Publication year - 1990
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1990.0011183x003000060035x
Subject(s) - biology , heat shock protein , ploidy , gel electrophoresis , genetic diversity , silver stain , staining , hsp70 , microbiology and biotechnology , genetics , gene , population , demography , sociology
Plants synthesize a set of proteins, called heat‐shock proteins (HSPs) under high‐temperature stress. These proteins may play a role in the genetic control of thermal tolerance in plants. The objective of this study was to determine if genotypic differences exist in the synthesis and accumulation of HSPs in diploid wheat Triticum monococcum L. Plants of three T. monococcum accessions were heat shocked at 37 °C in a controlled condition for 1 h and HSPs were labeled in vivo with 35 S‐methionine for 3 h. Analysis of HSPs separated by two‐dimensional (2‐D) gel electrophoresis showed qualitative and quantitative diversity of HSP synthesis in T. monococcum . In order to study HSP accumulation, plants were heat shocked at 37 °C for 8 h and 2‐D separated HSPs were visualized by silver staining. Both qualitative and quantitative diversity of HSP accumulation was observed. Optical density (visible light) of 34 HSPs ranged from 0.33 to 44.64 on silver‐stained gels and from 0.21 to 37.64 on labeled gels. Heat‐shock proteins unique to individual accessions were identified. The identification of genetic variability in HSP synthesis within diploid wheat provides a useful tool for genetic and physiological studies of the role of HSPs in higher plants.