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Linolenic Acid Content of Soybean Seed Estimated with 2‐Thiobarbituric Acid Test
Author(s) -
Bubeck D. M.,
Duvick D. N.,
Fehr W. R.,
Hammond E. G.
Publication year - 1990
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1990.0011183x003000040040x
Subject(s) - linolenic acid , soybean oil , biology , glycine , thiobarbituric acid , food science , gas chromatography , alpha linolenic acid , fatty acid , chromatography , linoleic acid , chemistry , biochemistry , polyunsaturated fatty acid , amino acid , lipid peroxidation , docosahexaenoic acid , oxidative stress
The linolenic acid content in soybean [ Glycine max (L.) Merr.] seed oil generally is determined by gas chromatography (GC). A more rapid method of comparing the linolenic acid content of soybean seed samples would be useful for screening a large number of lines in a soybean breeding program. The objective of this study was to determine if the 2‐thiobarbituric acid (TBA) reaction could be used as a rapid method for determining the relative differences in linolenic acid content of soybean seed samples. Sixty‐eight random F 2 plants from each of two populations were used to compare TBA scores with linolenic acid content as measured by GC. The correlations between TBA score and linolenic acid percentages were 0.71 for Cross 1 and 0.60 for Cross 2. The results indicated that the TBA test cannot be used as a substitute for GC analysis in evaluating soybean samples. However, the TBA test may be a useful method to rapidly eliminate genotypes with excessively high linolenic acid before GC analysis.