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Restriction Fragment Length Polymorphism in a Subclass of the ‘Mandarin’ Soybean Cytoplasm
Author(s) -
Grabau Elizabeth A.,
Davis William H.,
Gengenbach Burle G.
Publication year - 1989
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1989.0011183x002900060049x
Subject(s) - biology , restriction fragment length polymorphism , hindiii , mitochondrial dna , restriction enzyme , genetics , ecori , restriction fragment , microbiology and biotechnology , genome , restriction map , southern blot , cytoplasm , dna , gene , genotype , nucleic acid sequence
Mitochondrial DNA (mtDNA) from a number of soybean, Glycine max (L.) Merr., cultivars was examined by restriction endonuclease digestion to search for possible differences among cytoplasmic backgrounds. In these analyses it was observed that mtDNAs from certain members of the ‘Mandarin’ cytoplasm, represented by 19 cultivars maternally derived from ‘Lincoln’, were different from 38 cultivars derived from other cytoplasms. A 10.5‐kb (Kilobase) Hind III fragment that was absent from the Lincoln class of mtDNA was present in the mtDNA of the other cytoplasms tested. Use of the 10.5‐kb fragment as a hybridization probe in Southern analyses revealed the presence of a smaller 2.3‐kb Hind III fragment that was unique to the Lincoln‐derived mtDNAs. The 2.3‐kb Hind III fragment was cloned and used in Southern hybridizations of mtDNA to screen for differences among a large number of soybean cultivars. A portion of the 2.3‐kb Hind III fragment was found at a second location common to the mitochondrial genome of all cultivars tested.