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Polyploids from 2 x ‐4 x and 4 x ‐2 x Crosses in Red Clover
Author(s) -
Taylor Norman L.,
Berger Sandy
Publication year - 1989
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1989.0011183x002900010053x
Subject(s) - biology , polyploid , ploidy , red clover , sieve (category theory) , botany , horticulture , gene , genetics , mathematics , combinatorics
Efficient methods of producing tetraploids are important to red clover ( Trifolium pratense L.) breeders. An effort to widen the genetic base of tetraploid experimental lines via gametic nonreduction provided an opportunity to further examine this methodology. The objectives of this research were to further evaluate the frequency of triploid and tetraploid progeny from 2 x ‐4 x and 4 x ‐2 x crosses in red clover, and to examine the use of seed dockage sieves as a labor saving device in separating polyploid from diploid seeds. A few triploids were found among progenies from 2 x ‐4 x crosses in contrast to our earlier research, but the frequency was considerably lower than from 4 x ‐2 x crosses. Tetraploids were more frequent among progeny of the 4 x ‐2 x cross than progenies from 2 x ‐4 x crosses. Isolation of polyploid seeds was facilitated by sieving seeds and growing only those from the larger sieve sizes. The most efficient method of producing both triploids and tetraploids was by 4 x ‐2 x crosses rather than by 2 x ‐4 x crosses. However, a leaf mark or other gene marker is necessary to eliminate tetraploid selfs.