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Tetrazolium Test for Predicting the Seedling Vigor of Rice at Optimal and Low Temperatures
Author(s) -
Sung F. J. M.,
Chen J. J.
Publication year - 1988
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1988.0011183x002800060031x
Subject(s) - seedling , formazan , biology , cultivar , oryza sativa , embryo , agronomy , horticulture , botany , biochemistry , gene , microbiology and biotechnology
The amount of formazan extracted from tetrazolium salt‐treated embryos has been used to predict seedling vigor in some cereal crops. Our objective was to determine whether this technique could be used to evaluate the seedling vigor of rice ( Oryza sativa L.) cultivars grow at different temperatures. In this study, the genetic differences in formazan production capability of the embryo and its relationship to seedling vigor, as indicated by seedling dry weight accumulation within a 7‐d growing period, were examined in 21 cultivars. The results indicated that temperature treatments could play a crucial role in regulating the seedling growth response and formazan production capability in the embryo portion of the seed. Considerable variation in growth and formazan production level also existed in the rice cultivars. Seedling vigor was positively related to formazan production capability of the embryo, suggesting that the formazan extraction technique would be a simple and reliable method for screening rice cultivars for optimal and suboptimal temperature seedling vigor.