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A Hybrid Seed Production Method Based on Synthesis of Novel Linkages between Marker and Male‐Sterile Genes 1
Author(s) -
Jorgensen Richard A.
Publication year - 1987
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1987.0011183x002700040041x
Subject(s) - biology , sterility , hybrid seed , gene , transformation (genetics) , genetics , lycopersicon , mendelian inheritance , cytoplasmic male sterility , population , marker gene , genetic linkage , genetic marker , microbiology and biotechnology , botany , hybrid , sociology , demography
Mendelian male‐sterile genes are rarely used in hybrid seed production because pure stands of male‐sterile plants are difficult to obtain. We describe here a general method for hybrid seed production whereby linkage is synthesized between a male‐sterile gene and a marker gene through genetic transformation. Such linkage permits the production of nearly pure stands of male‐sterile plants. The method should be applicable in any plant species in which markers can be introduced into approximately random genomic locations. It is shown that the Agrobacterium transformation system can be used to synthesize tight, novel linkages between markers and male‐sterile genes in tomato ( Lycopersicon esculentum Mill.). In addition, a marker gene is identified that is potentially suitable for use in the proposed hybrid production system in several crops. This marker, IaaH , determines a dominant chemical sensitivity phenotype which, if linked in coupling phase with a male‐sterile gene, should allow chemical removal of fertiles from a population segregating for male sterility/fertility and chemical resistance/sensitivity.