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Effect of Abscisic Acid on Amino Acid Uptake and Effiux in Developing Soybean Seeds 1
Author(s) -
Guldan Steven J.,
Brun William A.
Publication year - 1987
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1987.0011183x002700040023x
Subject(s) - asparagine , abscisic acid , glutamine , glycine , biology , amino acid , proline , biochemistry , botany , gene
The role of abscisic acid (ABA) in regulating growth of developing soybean [ Glycine max (L.) Merr.] seeds is not fully understood. The objectives of this study were to characterize the effect of ABA on the in vitro uptake of asparagine and glutamine by isolated immature cotyledons in three soybean plant introduction (PI) lines with genotypic differences in seed growth rate and final seed weight. Cotyledons were incubated in uptake buffer solutions plus 14 C‐asparagine or 14 C‐glutamine and treatment concentrations of ABA. The ABA levels in the uptake solutions were 0, 10 ‐7 , 10 ‐6 , and 10 ‐5 M . The uptake rate of glutamine was approximately three times that of asparagine. Among PI lines, the heavy seeded line had a greater rate of asparagine uptake while the light seeded line had a greater rate of glutamine uptake. For asparagine, 10 ‐6 M ABA depressed uptake compared to the control. For glutamine, ABA enhanced uptake compared to the control at both 10 ‐6 and 10 ‐5 M . In an additional experiment, we observed no effect of ABA and K + on the release of labeled asparagine from excised soybean seed coats. These data indicate that amino acid uptake rates are genotypically dependent and may be influenced by ABA concentration.