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Effect of a Modified Potato Medium on Anther Culture of Wheat 1
Author(s) -
Marburger J. E.,
Sammons D. J.,
Schaeffer G. W.
Publication year - 1987
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1987.0011183x002700020048x
Subject(s) - stamen , biology , pollen , horticulture , solanum tuberosum , botany , solanaceae , tissue culture , in vitro , biochemistry , gene
Modification of a potato extract medium was undertaken to increase pollen embryogenesis from anthers of a hard red spring wheat ( Triticum aestivum L. cv. Chris). Whole potato tubers ( Solatium tuberosum L. cv. Kennebec) were separated into peeled tubers and peel from which extracts were prepared. A 3 ✕ 3 latin square design with three replications in time was used to compare media prepared from: (i) extract of whole potato tubers, (ii) extract of the peel only, and (iii) extract of peeled tubers. A 60% increase in anther induction resulted from the peeled tuber extract medium compared to the whole potato medium. Removal of the peel from tubers probably reduces the concentration of compounds that may be inhibitory to wheat embryogenesis during anther culture. Anther source, defined by spike section (basal, middle, and distal), was a second variable in the experiment but did not significantly affect embryogenesis.