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Early Development of Duplication‐Deficiency Ovules in Upland Cotton 1
Author(s) -
Contolini Carol S.,
Menzel Margaret Y.
Publication year - 1987
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1987.0011183x002700020045x
Subject(s) - ovule , endosperm , biology , anthesis , chromosomal translocation , botany , human fertilization , horticulture , meiosis , cultivar , agronomy , genetics , pollen , gene
Viable duplication‐deficiency (dp‐df) products are recovered from heterozygous translocations in Upland Cotton ( Gossypium hirsutum L.) with an overall frequency high enough to warrant their use in cytogenetic experiments. The frequencies of dp‐df's from most translocations, however, are considerably lower than predicted from meiotic configurations. Heterozygotes (Tt) of two translocation lines were studied to determine the period of development during which dp‐dfs were lost. In Tt2;14 2B‐l, which normally yields a high frequency of dp‐df's, the frequency of ovule failure was similar to that in TMI controls. Failure of ovules began at 5 days after anthesis in Ttl0;ll 2785. A high percentage of ovules from Ttl0;ll at 5 days postanthesis were found with intact synergids and polar nuclei, indicating that failure may occur daring fertilization. Endosperm failure may also be responsible for some dp‐df loss, because, in 4% of ovules from Ttl0;ll, nucellar cells filled the interior of the ovule and obliterated the space normally occupied by embryo and endosperm. Attempts were made to enhance the recovery of dp‐dfs by culture of 2‐day‐postanthesis ovules. In both lines, the frequency of dp‐dl's was significantly higher ( P = 0.001) in the cultured progenies than in controls. In ovulo culture also decreased by 50% the time required to obtain seedlings.