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Improvement in Callus Growth and Plantlet Formation in Creeping Bentgrass 1
Author(s) -
Blanche Fe C.,
Krans J. V.,
Coats G. E.
Publication year - 1986
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1986.0011183x002600060035x
Subject(s) - plantlet , callus , suspension culture , biology , botany , horticulture , somatic embryogenesis , tissue culture , cell culture , in vitro , biochemistry , genetics
Optimizing callus growth and plantlet formation from somatic callus is advantageous in the recovery of desirable variants using in vitro cell selection methods. Using 24‐week‐old ‘Penncross’ creeping bentgrass (‘Agrostis palustris Huds‘.) stock callus (SC), callus growth and plantlet formation were evaluated from plated SC previously cultured in suspension for 12 and 24 days and SC not previously cultured in suspension. In addition, callus growth and plantlet formation were evaluated from SC plated at aggregate sizes of 0.5‐ to 1.0‐mm and 0.25‐ to 0.5‐mm diam and aggregate densities of 1, 3, and 5 mg.50 mm −2 . Callus growth was twofold greater from SC previously cultured in suspension than from SC not previously cultured in suspension. Plantlet formation was inhibited from SC previously cultured in suspension (<10 plantlets/dish) compared to SC not previously cultured in suspension (250 to 450 plantlets/dish). Plated SC aggregates of 0.5‐ to 1.0‐mm diam generally had greater growth than aggregates 0.25‐to 0.5‐mm diam. At both aggregate size dimensions, greatest growth occurred from SC plated at 1 mg.50 mm −2 plating density. Aggregates 0.5‐ to 1.0‐mm and 0.25‐ to 0.5‐ mm diam had the greatest plantlet formation at 1 mg.50 mm −2 and 1 and 3 mg.50 mm −2 densities, respectively.