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A New Technique for Crossing Mungbeans 1
Author(s) -
Cupka T. B.,
Edwards L. H.
Publication year - 1986
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1986.0011183x002600040043x
Subject(s) - emasculation , biology , pollination , pollen , embryo rescue , stamen , agronomy , self pollination , horticulture , botany
Improved crossing techniques are needed to exploit the genetic potential of mungbean [ Vigna radiata (L.) Wilczek]. In our technique, only the tip of the bud is opened to expose the stigma and style. After the anthers are gently removed with forceps, the emasculated bud is then ready to be crossed. When pollination is completed, cellophane tape is placed over the opening to seal the bud and the process is complete. An experienced technician can complete an emasculation and pollination in 1 min. The success rate using this technique averaged 60% with an average of six seeds per pod per successful cross. This technique differs from those presently being used in other legume crops because most other crossing techniques open the entire dorsal edge of the bud in order to make an emasculation and pollination. This technique appears to reduce moisture and temperature fluctuations within the bud and reduces bud abortion subsequent to artificial pollination.