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Genetic Analysis of a Null‐Allele for Lipoxygenase‐2 in Soybean 1
Author(s) -
Davies C. S.,
Nielsen N. C.
Publication year - 1986
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1986.0011183x002600030003x
Subject(s) - biology , lipoxygenase , locus (genetics) , allele , isozyme , null allele , genetics , gene , genotype , glycine , microbiology and biotechnology , enzyme , biochemistry , amino acid
Soybean [ Glycine max (L.) Merr.] seeds contain at least three isomeric forms of lipoxygenase. We have identified a soybean line, PI 86.023, which is homozygous for a gene that confers the absence of the lipoxygenase‐2 isozyme. Analysis of electrophoretic banding patterns of seed extracts from the F 2 and F 3 progeny of crosses between PI 86.023 and breeding lines containing null‐alleles for lipoxygenase‐ 1 and ‐3 indicated that this gene was tightly linked to the Lx 1 locus and independent of the Lx 3 locus. We propose that the dominant allele for this gene be called Lx 2 and the recessive null be designated lx 2 . Seeds were identified that lacked both lipoxygenase‐2 and ‐3, but none was found that lacked both lipoxygenase‐1 and ‐2. The lx ‐ 2 null‐allele will be useful for investigating the effect of lipoxygenase‐2 on the production of off‐flavors during processing of soybean products.